Phosphorylated H2AX (designated as γH2AX) rapidly accumulates over megabase domains at the sites of DSB 8 and, therefore, can be microscopically visualised as discrete nuclear foci. These foci can

photo bleaching (FRAP assays), gene knockdown using RNAi technology, DNA damage evaluation in single cell by gamma-H2AX staining and Comet assays

Following radiation exposure, histone H2AX is rapidly phosphorylated by ATM and/or DNA-PK kinases at or near the vicinity of DNA DSB sites to form γ-H2AX [ 14 ]. HT gamma -H2AX Pharmacodynamic Assay Summary. Enables the quatification of double strand DNA breaks through the detection of gamma-H2AX in peripheral blood mononuclear cells, cultured cells, and tissue biopsies. H2A.X Phosphorylation Assay Kit (Flow Cytometry) The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. - Find MSDS or SDS, a COA, data sheets and more information. Abingdon, UK & Lake Forest CA, USA — AMSBIO announces the first commercially available gamma H2AX Pharmacodynamic assay kit for the study of double strand DNA breaks through the detection of gamma H2AX – a phosphorylated histone historically proven as a highly specific and sensitive molecular marker for double strand DNA damage detection. The gamma-H2AX assay has up to now not been applied in this context, and it is a promising tool for investigating the early cellular response to mixed beam irradiation.

The prognostic value of gamma-H2AX is indicated in certain cancer types, such as breast or endometrial cancer, but further investigation is needed to establish gamma-H2AX as a prognostic marker. 2016-01-20 AMSBIO announces the first commercially available gamma H2AX Pharmacodynamic assay kit for the study of double strand DNA breaks through the detection of gamma H2AX - a phosphorylated histone historically proven as a highly specific and sensitive molecular marker for double strand DNA damage detection. This new assay has been developed for anti-cancer drug screening, basic research and Abstract Purpose: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma-H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise.

007954116. The sensitivity of the γ-H2AX assay is limited by the variability of foci levels in untreated cells, and is therefore cell type-dependent.

We quantified gamma-H2AX foci manually and automatically. In addition, total gamma-H2AX activation was determined by flow cytometry. For all chemicals the cytotoxic dose response was assayed by a metabolic cytotoxicity assay.

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H2A.X Phosphorylation Assay Kit (Flow Cytometry) The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. - Find MSDS or SDS, a COA, data sheets and more information.

To substantiate the similarity observed between the human and minipig we show that minipig fibroblast radiosensitivity was similar to that observed with human fibroblasts. A number of ELISA Kits are available that target gammaH2ax, an alias of the protein, H2A histone family member X. The human protein, encoded by the gene H2AFX, is a member of the Histone H2A family. The protein's cellular localization is indicated to be nuclear. Additional synonyms or past names for this protein may include: H2A.X, H2A/X, H2AX. 2018-06-01 · Gamma-H2AX assay has proved useful for detecting DNA damage at doses of radiation down to 1 mGy and it is said to be 100 times more sensitive than other methods , . Reportedly the gamma-H2AX can be quantified either by immunoflourescence or flow cytometry [14] . 2013-11-29 · Additionally, although our data clearly indicate a non-cumulative response of the γ-H2AX phosphorylation and stress the impact of split dose exposures on the DNA repair machinery, the clinical relevance of these findings will have to be validated for the specific cell lines and 3D/in-vivo models in which the γ-H2AX assay is proposed as a biomarker.

2009). Conversely, EMS appeared strongly positive in the comet assay but only mildly in the gamma‐H2AX immunofluorescence. These observations suggest that the two methods could complement each other for DNA damage analysis, where gamma‐H2AX staining allows the detection of tissue‐specific effects in situ. This modification, called gamma-H2AX (phospho-Ser139), triggers cell cycle arrest and DNA damage response repair, which makes this assay particularly appropriate for studying genome stability, cell cycle, DNA repair, and more broadly as a biomarker for cancer and for aging process investigations.
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This modification, called gamma-H2AX (phospho-Ser139), triggers cell cycle arrest and DNA damage response repair, which makes this assay particularly appropriate for studying genome stability, cell cycle, DNA repair, and more broadly as a biomarker for cancer and for aging process investigations.

2013-11-29 Gamma-H2AX assay is an immuno-fluorescence experiment that enables detecting the location and number of DNA double strand breaks (DSBs) in cells. Assuming that a single gamma-H2AX focus corresponds to a single DSB, one can convey the severity of cellular damage by the number of gamma-H2AX foci per cell (FPC) [1].
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We quantified gamma-H2AX foci manually and automatically. In addition, total gamma-H2AX activation was determined by flow cytometry. For all chemicals the cytotoxic dose response was assayed by a metabolic cytotoxicity assay.

Additional synonyms or past names for this protein may include: H2A.X, H2A/X, H2AX. 2018-06-01 · Gamma-H2AX assay has proved useful for detecting DNA damage at doses of radiation down to 1 mGy and it is said to be 100 times more sensitive than other methods , . Reportedly the gamma-H2AX can be quantified either by immunoflourescence or flow cytometry [14] . 2013-11-29 · Additionally, although our data clearly indicate a non-cumulative response of the γ-H2AX phosphorylation and stress the impact of split dose exposures on the DNA repair machinery, the clinical relevance of these findings will have to be validated for the specific cell lines and 3D/in-vivo models in which the γ-H2AX assay is proposed as a biomarker.